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KMID : 0613820060160020240
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Journal of Life Science
2006 Volume.16 No. 2 p.240 ~ p.244
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Development of a Highly Efficient Isolation Protocol for Mitochondrial DNA and RNA Using Small Scale Plant Tissues
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Kim Kyung-Min
Lim Yong-Suk
Shin Dong-Il
Sul Ill-Whan
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Abstract
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We present a fast and simple protocol for purification of mitochondria, mitochondrial DNA, and RNA from small amounts of tomato leaves. This method uses a high ionic strength medium to isolate mitochondria and extract mitochondrial DNA and RNA from a single preparation and is easily adaptable to other plant species. Mitochondria was confirmed by MitoTracker. The mitochondrial DNA was not contaminated by plastid DNA, was successfully used for PCR. Similarly, the isolated mitochondrial RNA was not contaminated only slightly contaminated (leaves) by plastid RNA. RNA prepared according to our method was acceptable for RT-PCR analysis
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KEYWORD
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Tomato, mitochondria, mitochondrial DNA, mitochondrial RNA, MitoTracker
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